ExCel Matrix Biological Devices (P) Ltd.
A Biodegradable Microcarrier
Product Design Concept
Microcarrier was developed in 1967 for viral vaccine manufacturing. It remains as most reliable large scale manufacturing option till date. Microcarrier method can be scaled up easily, however cell yield (number of cells/mL) remains same as conventional methods like Roux bottle and roller bottle. Low product yield as consequence, very often do not justify large investment on bioreactors and utility needed to adopt microcarrier technology for viral vaccine manufacturing.
To improve cell yield by increasing the amount of microcarrier/mL is limited due to increase in collision among microcarrier, bioreactor impeller, baffles and walls leading to cell death.
Porous microcarrier, PMc® on the other hand protects cells from collisions as it is soft and allow cells to grow inside the cavities of large inter-connected pores. It is therefore possible to achieve more cells and enhance product yield.
Surface area of PMc® is also significantly higher than normal microcarriers to allow high density culture under perfusion mode. As PMc® is exceptionally susceptible to trypsin, cells can be harvested without any carried over micro-particles during inoculum development.
Collagenous and ECM based porous scaffolds are quite suitable for tissue engineering. PMc® is designed for regenerative medicine (tissue engineering and stem cell) research and application development. PMc® is made up of extra cellular matrix like amniotic membrane, a well proven biomaterial for primary cell phenotype maintenance and clinical application point of views. PMc® has merged the advantages of microcarrier and bioreactors on single platform through ECM Analog® technology.
Please discuss with our experts your unique cell culture requirements and explore options beyond conventional products.
PMc® is available in two variety.
- Type I (High density particles suitable for submerged culture)
- Type II (Low density particles suitable for perfusion culture)
|PMc® I||PMc® II|
|· Density > Culture media|
· Sizes 50 to 100 µm
· Sensitive to Microcarrier Concentration, Agitation and O2
|· Density ≤ Culture media
· Sizes 50 to 100 µm
· Float in medium without agitation.
· Little or no Limitations for High Density Culture
· Leads to low shear and O2 limitation.
· Easy perfusion from the bottom of culture vessel.
- PMc® is gamma irradiated and available in ready to use packing.
- PMc® is available in two types of ECM material.
- Collagen Type I
- Amniotic ECM (coming soon)
- Customizable Extra Cellular Matrix for 3D culture of specific cell types.
- High Density cell culture for viral vaccine manufacturing.
- Recombinant protein manufacturing.
- Culture inoculum preparation at high density.
- Culture stock maintenance at lower passage numbers.
- In situ cryo preservation of 3D cultured cells.
- Regenerative medicine and Stem cell research.
- Artificial tissue regeneration (Skin, Cartilage etc.).
- PMc® is transparent for easy microscopic observation without phase contrast.
- Cells can be stained with methylene blue in Ca++, Mg++ free PBS to increase contrast against PMc® matrix with or without fixation.
- Harvesting of cells by Trypsin in few minutes.
Hardware for culturing PMc®
- Tube rotor
- Spinner flasks
Accessory equipments are quite inexpensive and available on request (for research purpose only).
Porous Microcarrier PMc® is for research purpose only.